6/26/2023 0 Comments Watch the vault 2021![]() Alongside a cheesy suiting-up montage, the third act is loaded with uninspired twists and the obligatory how-they-did-it reveal. The action-thriller offers a fun and unique twist on the classic heist movie, though it cannot help but indulge in some of the genre’s worst habits. ![]() ![]() When it’s time for the climax, the main robbery itself is smart, suspenseful and exciting – just the way it should be. Balagueró keeps the events unfolding at a steady pace, which keeps viewers enthralled in the action as the crew’s plan comes together. What follows is a series of slickly executed sequences that see the crew get ever closer to solving their conundrum, with one early infiltration mission establishing the stakes and setting nicely. His crew have most of the details worked out except for the secrets of how the vault works, and that’s where Thom comes in. After turning down a dozen high-paying corporate jobs, he’s approached by self-proclaimed treasure hunter Walter (Liam Cunningham), who wants his aid on a salvage mission – the job: to recover artefacts they believe will lead them to Sir Francis Drake’s long-lost treasure from the Bank of Spain. The plot centres around Thom (Freddie Highmore), a genius engineer freshly out of university. While this picture sustains the action and suspense throughout, it unfortunately falls into a number of genre trappings. The director uses every frame and edit of his latest venture to move the narrative forward while forging a genre piece that pays homage to the old classics Italian Job and Oceans Eleven. Jaume Balagueró’s (one half of the team behind Rec) The Vault (aka Way Down) is a suave heist thriller that comes together like a well-oiled machine.
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6/26/2023 0 Comments Elf on the shelf ideas![]() What's a good The Elf on the Shelf gift?Įlves come with everything they need to get settled in their new home, but feel free to add some The Elf on the Shelf clothes or accessories to make the experience more fun! These additional items might even inspire some more elf magic. There's really no harm in starting earlier-your child just might not be as engaged at an earlier age. Of course, if there are older siblings in the house, the tradition will start even sooner than that. It depends on the child, but we recommend three years old, or as soon as he or she starts to understand the magic of Christmas. Elves are great listeners! What's a good age to start Elf on the Shelf? Luckily, we've compiled a list of the best The Elf on the Shelf names! The children should be encouraged to tell their new elf any wishes or secrets. If this is the elf's first trip to your house, you'll have a bit of work to do! Your Elf needs a name, for starters. So, traditionally, during Scout Elf Return Week (November 23-December 2), the elf arrives. When you first get The Elf on the Shelf, he or she comes with an illustrated Christmas book that explains the whole backstory. No exceptions! How do you introduce The Elf on the Shelf for the first time? Scout elves arrive during Scout Elf Return week and head back to the North Pole on Christmas Eve. What are the two rules of The Elf on the Shelf?Įlf 101: The children should not touch the elf, or he will lose his magic. These ideas are inexpensive and often free, so you don't have to worry about another expense this time of year.īefore you get started, here's what you need to know about this popular Christmas tradition. ![]() But whatever the case in your house, it's best to be prepared so you're not searching for last-minute elf ideas (we've all been there!). Some elves are funny and some are a bit naughty, and others like participating in family Christmas games. But when the Scout Elves return, it's time to be prepared with all of the best The Elf on the Shelf Ideas for 2022. This mischievous character's return to our homes marks the beginning of holiday excitement, right along with the debut of advent calendars and the writing of Merry Christmas wishes in our cards. But one important date looms large each year: the return of The Elf on the Shelf. When the holiday season arrives in our homes, we have several big dates marked on the calendar, from Thanksgiving to Christmas to New Year's. 6/26/2023 0 Comments Smarts registration![]() See further information about cancellations due to Covid-19 here. You waive your 14-day withdrawal right when you register for this conference.Substitute participants will be accepted at any time – a name will be required.Non-arrivals will be non-refundable for any reason.Please note that registration is binding. If you wish to participate in person in Copenhagen and in the conference dinner, additional fees are added to the basic conference fee, when you select these options in the form.Īll prices are inclusive of 25% VAT. If you wish to participate online, no further fees are added. To enable you to participate in the community and to offer you and the other community members the best service. Registered farmers are provided with a personalized dashboard. When you register, you pay a basic conference fee. SMART Registration ID can be utilized to avail various services from department in future. ![]() Additional fee for conference dinner (Copenhagen): 100 EUR.Normal fee: 475 EUR (attendance in Copenhagen) / 375 EUR (virtual attendance).Early registration (for presenters with accepted abstracts): 375 EUR (attendance in Copenhagen) / 275 EUR (virtual attendance).Your registration process will be handled securely on the platform. Please click the below button to register for the Conference. The event portals will be hosted on PheedLoop, one of the leading event management platforms. Questions about the collection of this information may be directed to REGISTER HERE This information will be used for the purpose of events management and payment processing only. Please note your personal information is collected under the authority of section 26(c) of the Freedom of Information and Protection of Privacy Act (FIPPA). No refund – Cancellation request received after JPRIVACY NOTICE REFUND SCHEDULEġ00% – Cancellation request received by May 31, 2022ĥ0% – Cancellation request received after and no later than June 30, 2022 There will be no refund after June 30, 2022. You will receive a full refund if it is cancelled on or before and 50% refund from June 1 until June 30, 2022. If you wish to cancel your registration, please send your request by email to soon as possible. The primary contact person or signatory for your company receives an email from GEP SMART with a link to register by filling in the Primary Registration Form (. It is recommended to use credit card for payment, but if you need to pay by EFT, please request for the wire instruction by email to In order to receive the early bird discount, your transfer should be received by us by June 20th.Registration fee includes access to all sessions, conference mobile app, the welcome reception, lunches, refreshments, and the networking dinner on Aug 8th.We accept Visa, Mastercard and American Express. Secure online credit card payments are handled by Stripe.Globe replied to some netizens, saying they. Tuesday, the first day of the mandatory SIM registration. All conference registration fees are quoted in Canadian dollars (CAD). Don't miss out on epic offers like Smart Power All 50 and register your Smart SIM para hindi ma-SIM DEAD.With Power All 50, get 5GB for all. Both Globe and SMART registration links showed a 'Bad gateway' prompt as of 6:30 a.m. 6/26/2023 0 Comments Cinnamon rumped seedeater![]() Morelet's/Cinnamon-rumped Seedeater ( Sporophila morelleti/torqueola), version 1.0. Birdforum thread discussing id of White-collared Seedeater.The eBird/Clements checklist of Birds of the World: v2021. Song high-pitched and canary-like, a clear sweet sweet sweet cheer cheer cheer followed by various trills. Three to four eggs are pale blue to greenish blue with speckles, blotches, or spots of deep brown or black. Will jump into air to catch flying insects.īegins in late March and continues into summer in Southern Texas begins in April in Northern Central America. Open grassy areas, pastures, roadsides, weedy fields, moist savanna, and marshlands with tall reeds.Įats mainly grass seeds, augmented with a few berries and insects. mutanda in which some males have a black throat is considered a synonym of nominate. Caribbean slope of southern Mexico (Veracruz) to extreme western Panama and Pacific slope from southern Mexico (Chiapas) south to western Panama (Chiriquí).morelleti:Black breast band of male is complete black on back is more extensive and has white crescent below eye. Southern Texas (lower Rio Grande Valley) to eastern Mexico (Veracruz).sharpei:Black breast band of the male is reduced to black mottling at the sides of the breast. This is a polytypic species consisting of two subspecies : The former White-collared Seedeater has been split into this species and the Cinnamon-rumped Seedeater. Eastern Mexico and the far southern Rio Grande Valley in Texas additionally along the Caribbean slope south to western Panama. Female Variable Seedeater does not have wing bars, and is more olive in color. The male Variable Seedeater is almost all black. Female and winter male Ruddy-breasted are more similar to female Morelet's, but Ruddy-breasted is smaller, with a paler bill and more contrasting wing edgings. Within its range, the male Ruddy-breasted Seedeater in breeding plumage has a two-toned plumage, gray above and ruddy below. ![]() The second has underparts white to pale buff.Ĭinnamon-rumped Seedeater of West Mexico is similar but lacks wing-bars. The black bib/breast band and the white collar in the male varies between weak in subspecies sharpei and much stronger in e.g., moreletti. ![]() Its crown is still mottled with dark, but is otherwise similar to female.įemales are much less patterned, and are buffy brown above with two white wing bars. The winter male keeps the blackish wings, whitish wing bars and white spot at the base of the primaries. The breeding male has a dark head and back, a variable white collar and black breast band, variably buff belly, double white wing bars and white rump. This is a small finch with a short, stubby dark bill and curved culmen and short tail. Crooked Tree Village, Belize, 26 January 2011 6/26/2023 0 Comments Rolling tumbleweed gifŁyse is a well-known centre for the culture of the Kurpie people who settled at the northern edge of Mazovia in the 17th century. The Radom Village Museum, meanwhile, has recreated two complexes: a village of peasant cottages and a manor complex with manor houses, farm labourer quarters, a smithy, a granary, a henhouse, and even a toilet.Įaster palms from the Kurpie region, presented in the village of Łyse on Palm Sunday, can be up to 10 metres tall. You can spend the night here, enjoy horse riding and try your hand at harvesting. In addition to genuine peasant homesteads, this open-air museum also features an inn, a church with belfry, and even a reconstructed manor house with garden. Other interesting routes include the Mazovian Literary Trail, Industrial Mazovia, Mazovia in Films, and the Great Lithuanian Route.Ī visit to the Museum of the Mazovian Countryside in Sierpc is perfect for learning about historical rural rituals and folk customs. invasion, 1655-1660), and the fortress went through its first siege from Russian forces after Napoleon’s retreat from Moscow, followed by the November Uprising of 1830-1831, and both world wars. Construction began in the times of the Swedish Deluge (i.e. The impressive walls of the Modlin Fortress, today a museum on the Battle of Warsaw 1920 Trail, cast a stunning reflection on the waters of the Vistula. The tragedy of the Holocaust is exhibited at the Treblinka Museum, the site on which the German World War II Extermination Camp during World War II murdered about 780,000 Jews, mainly from the Warsaw Ghetto, the largest ghetto in Europe. Płock is home to the Museum of Mazovian Jews. The remains of old shtetls, synagogues, Jewish cemeteries and sites of remembrance can be found in locations such as Otwock, Karczew, Góra Kalwaria and Przysucha. The Jewish heritage that has survived to this day shows the enormous contribution made to Polish culture by the centuries-long presence of a large Jewish diaspora on the River Vistula. The “poet of the piano”, whose technique was incredible and who composed his music when Poland was partitioned by three foreign powers, declared his patriotism through works, especially preludes and mazurkas, which drew upon his native Mazovian folk music. The Chopin Trail takes you on a journey to locations where Fryderyk spent the first twenty years of his life - Żelazowa Wola, Warsaw, Sanniki and Brochów. Mazovia also offers a number of other themed tourist routes. To see them, you can follow marked routes – the Duke Konrad of Mazovia Route which focuses on the progenitor of the dynasty, the Route of Duchess Anna or the Route of the Siemowits – and admire Gothic brick castles and churches full of fascinating legends. Their legacy includes over a hundred towns, such as Zakroczym, Czerwińsk, Wyszogród, Serock, Przasnysz, Ciechanów, Czersk and Sieciechów. Their story is presented on the Dukes of Mazovia Route. This lateral branch of the Piast family built its state practically from scratch, generation by generation, and maintained its independence for three centuries. The key to that treasure is the fascinating history of this land, today the heart of Poland and home to the country’s capital, Warsaw.įans of Sapkowski’s The Witcher series will recognise the wyvern (a type of dragon with just two hind legs and the wings of a bat) that is found on the seals and banners of the Mazovian dukes in the Middle Ages. This flatland striped with meadows, fields and forests, with spots of increasingly colourful towns and villages, the River Vistula cutting across it like a ribbon, is a region full of tourism treasures. ![]() Flat, its great expanse – under streams of swooshing stars, under a river of pines…” This is how poet Krzysztof Kamil Baczyński described the region in a few simple words. km and is Poland’s largest province in terms of both area and population. ![]() ![]() Mazowieckie province covers an area of over 35,500 sq. ![]() Mazowieckie province lies in central-eastern Poland and shares a border with six provinces: Warmińsko-Mazurskie, Podlaskie, Lubelskie, Świętokrzyskie, Łódzkie and Kujawsko-Pomorskie. To the south of the capital lies a health resort, and to the north-west Kampinos National Park, not to mention the surrounding localities explored via the winding roads of Mazovia… Filled with attractions for the active and history-loving tourist, with traces of Chopin’s life and work, this is the largest province and home to Poland’s capital city - Warsaw, whose Old Town is a UNESCO-listed heritage site. 6/26/2023 0 Comments Michaels porthole oceanside![]() no disappointments with the food or service. most importantly, lobster bisque is a must try. Disgusting Rolando 6 years agoįirst time here with my family. Aside from paint nite, there were 6 people in the place. What used to be a very tired looking Michaels Porthole is now Jessies On The Water, owned by Craig Ozzie. Said they don't take Groupons on "event nights" (they were having a paint nite.) It said nothing of this in the fine print. Ranked 53 of 121 Restaurants in Oceanside. (He did not) he said "people do this all the time" as if I were trying to get away with something. Said he told me on the phone they only accept Groupon after 7:30. Get delivery or takeout from Michaels Porthole at 92 New Street in Oceanside. the atmosphere is definitely blue jeans and t-shirt and the location is industrial and dirty. this place is better in the summer outdoors. Jim Frasier of Oceanside, California, wants to know if there's a wreck in. skirt steak tid bits very good, salmon a little overcooked, shrimp was very good. IN PARADISE Michael Lawrance 1996 REEF Field Surveys Take a dive vacation that. Service and food was terrific, very friendly atmosphere. On the water so in the summer there's a large newly renovated deck to enjoy. Cole slaw was not good at all Maryann 6 years agoįood, drinks, portions, & prices were great! Sharon 6 years agoįood was delicious and nice ambiance Flora 6 years ago Michaels Porthole is a great place to come for fun, cocktails and food, in a family run atmosphere. Would recommend that you try this place at least once. Service tends to be a little slow but that hasn't stopped us from going back. Located in an industrial area surrounded by water. Michaels Porthole (1. Dining out, listen to your favorite band, have a drink, paintnite and parties. Definitely need to go there more often! Reni 6 years ago Learn more about Chase on 3285 Long Beach Rd in Oceanside, New York (bank hours, contact details and. Michaels Porthole Restaurant and Bar in Oceanside near the East Rockaway border. to 9 a.m., teachers get a free breakfast entrée plus a Taquito with cheese, Breakfast on a Bun or Honey. ![]() I always loved this place, but hadn't been there in a while. Educators can get free breakfast entrées from May 8 to May 12. Atmosphere is great, service very good, drinks are heavy handed and food isn't good Laurie 6 years ago 6/25/2023 0 Comments Dont starve together stagehand
![]() Gene Set Enrichment Analysis with ClusterProfiler.NGS Sequencing Technology and File Formats.Next-Generation Sequencing Analysis Resources.Here’s an overview of the various ways you can compare protein and DNA sequences with MacVector. The tool to which most people first turn to for aligning sequences is the Multiple Sequence Alignment tool. This allows you to align multiple DNA or protein sequences using either Muscle, Clustalw or T-Coffee. This functionality is most suited for protein alignments, or for nucleic acid sequences where you are interested in examining phylogenetic relationships. Remember that you can also align DNA sequences based on their protein translations.Ĭonfirming a small sequencing project against a reference.Īt some point all molecular biologists have to verify that a sub cloning procedure has worked or a new construct is correct. The Align to Reference tool is perfect for quickly verifying a set of sequencing reads against a referenceĪligning cDNA/mRNA sequences against a genomic template.Īlign to Reference can also be used to align cDNA clones against a genome sequence. Click ALIGN with the algorithm set to cDNA ALIGNMENT.The steps are similar – use the genomic sequence as the reference, then add one or more cDNA clones to the alignment. Using a Dot Plot to quickly compare two sequences.ĭot Plot is great for identifying weak regions of similarity between two sequences. Dot Plots are also the best way of identifying sequence rearrangementsĬompare Genomes will compare two related annotated genomes (or smaller sequences) to identify and list, identical, similar and weakly similar features along with missing features. Use this to identify and align a sequence to the databases at the NCBI using the BLAST algorithm. To download and save hits, select hits and click TO DISKĪligning a sequence against a folder of local sequencesĪlign to Folder allows you to scan a local folder full of sequences and align them using the FastA alignment algorithm.To retrieve hits select the hits and click TO DESKTOP.You can download any hits directly to your Desktop including all annotations. Its kind of like a local BLAST, but more sensitive. Choose a folder of sequences and click ALIGN. ![]() This requires our optional Assembler add-on. Use this if you want to align ten or more DNA sequences with the idea of assembling them into a longer sequence with a consensus (de novo) or for aligning reads against a reference (resequencing). Add your sequencing reads and also any reference sequences.Primer Database.lets you automatically map your primer collection to any sequence you open. Go to MACVECTOR > PREFERENCES > SCAN DNA FOR.MacVectorTip: How to copy a specific short amino acid translation of a sequence.Simulating DNA electrophoresis in agarose gels using MacVector’s Agarose Gel tool.MacVectorTip: How to find Restriction Enzymes that only cut outside of a specific region.Getting Started with MacVector: An overview of primer design workflows in MacVector.PCR primer sets were designed to target nrfA, the gene encoding the pentaheme nitrite reductase NrfA that catalyzes the nitrite ammonification step in the process of dissimilatory nitrate reduction to ammonium (DNRA).Melissa Caimano on HOW DO I video guides to common molecular biology workflows.admin on HOW DO I video guides to common molecular biology workflows.mariam abdelmalak on Major release details – Summary.Brian on Designing primers and documenting In-Fusion Cloning with MacVector.Chris on Designing primers and documenting In-Fusion Cloning with MacVector.MacVectorTip: Designing Primers for Gibson Assembly.MacVectorTip: Simulating mixed plasmid populations in agarose gels. Details of the nucleotide alignments of the primer target regions of 271 nrfA sequences from reference genomes representing 18 distinct clades of NrfA are shown here along with validation of application to PCR-based methodology including the use of amplified fragment length polymorphism (AFLP) profiling and Illumina platform amplicon-based sequencing of environmental samples and selected reference strains. Summary data tables illustrate the specificity of forward primers nrfAF2awMOD and nrfAF2awMODgeo when paired with the new reverse primer nrfAR1MOD in relation to consensus target reference sequences associated with members of 18 NrfA clades. Specificity of the new primers to nrfA sequences in environmental samples is shown in AFLP analysis and amino acid-translated amplicon sequences obtained with the new primer sets. We also provide sequence alignment files of the full length nrfA genes, PCR reference amplicon alignment, NrfA amino-acid alignment and NrfA translated PCR amplicon-amino acid alignment. 6/25/2023 0 Comments Vmd installOnce the system boots into the OS, install the SetupRST.exe file downloaded in Step 1. This will install the Windows driver and give the opportunity to download the Intel® Optane™ Memory and Storage Management application from the Microsoft Store* for management of RAID/Intel® Optane™ memory volumes.Step 4: Install the Intel ® RST driver in the OS Select the drive for the OS installation.Drivers/VMD/ within the driver files copied in the USB key media. Browse to the location of the iaStorVD.sys file.The VMD driver will need to be installed to detect the drives being managed by Intel® RST under the Intel® VMD Controller. During the OS installation process, click Load Driver.Refer to Step 1 if the USB key media does not have the driver files copied. Insert the USB key media where the driver files were copied.Start the Windows OS installation process.The Boot Option setting should be UEFI before proceeding. The Legacy Boot Option will result in failures once the OS is installed with the Intel® RST software. Save the changes and exit. Intel® RST functionality requires a UEFI OS installation.Select SATA Controller to enable this to be managed by the Intel® VMD controller.Enable the available storage ports that contain the devices to be managed by the Intel® RST driver.Image below is for reference purposes only. Refer to the system manual or vendor's support site for specific information for the platform being used. Locate the VMD Setup Menu for the platform. Available settings and location in the menu can vary.Step 2: Enable the Intel ® VMD controller in BIOS The file iaStorVD.sys that will be used later on is located here. ![]()
6/25/2023 0 Comments Sifu platforms![]() One against all, you have no allies, countless enemies, and a mysterious amulet to bring you back to life every time you die. Whether you choose to play as a male or a female character, in Sifu, you will ponder that question on your path for revenge, hunting down your family’s assassins. With 5 new game modes, 9 dynamic locations and 45 challenges, gear up for spectacular combats, brutal opponents, and endless opportunities to refine your moves. Sifu’s Xbox release will only improve the market potential of the game and further its success, especially if it lands on Xbox Game Pass.Itching for some more good ol' Kung Fu action? The free Arenas expansion adds multiple hours of gameplay that will put your Kung Fu to the test. The game has been widely acclaimed by critics and players, and it’s safe to say it’ll be welcome on the Xbox platform. Sloclap had an amazing amount of success with Sifu’s release, selling over a million copies in three weeks, which is incredible for the studio’s sophomore endeavor. ![]() There are similarities between the release of Sifu and Absolver, which remained exclusive from August 2017 to January 2019. Sifu’s release on Switch in November 2022 may mean that Sony and Sifu’s publishers agreed a deal to allow it to come to the Switch after six months, and the Xbox after a whole year had passed. It’s safe to assume that the game will have around one year of timed exclusivity before being available on other consoles, meaning the earliest Sifu can appear on Xbox Series X & S and Xbox One is February 8th, 2023. Sifu was released for PS4, PS5, and PC on February 8th, 2022. Sifu Series X & S and Xbox One Release Date The second ending will occur during your character’s battle with Yang at the location where your vision is set. The first ending will repeat your character’s previous vision. Sifu has two different endings that depend on the choices you make in-game. Now, the player may choose to spare any person they want to. When Yang has been killed, the player’s character has a vision about Yang’s past, and the talisman sends them back to before the revenge spree. They swear revenge on those that murdered their father and go after each martial artist, killing each of them. Sifu is set in modern-day China, and players take the role of the only child of a sifu who was murdered by a disgraced former student and four other martial artists. Eventually, a player will be unable to resurrect, and the game will end. As the player becomes older, their strikes will become more powerful, but their maximum health will decrease. When the player dies in the game, they are magically resurrected at the spot of their death, having aged several years. The game is inspired by Bak Mei kung fu and has over 150 unique attacks ranging in skill level. Sifu is an action beat ‘em up style game that’s played from a third-person perspective. Here’s everything you should know about Sifu coming to Xbox. It’s safe to say that, while Sifu may not be available on Xbox One or Xbox Series X & S just yet, it should be released in 2023. In fact, Sifu has been quite a success for its developer, Sloclap. The expertly designed combat that Sifu contains has been raved about by critics and players alike. ![]() Games that are timed exclusives aren’t new in the industry, as both Xbox and PlayStation have done them and will continue to release timed exclusives in the future. ![]() Sifu is a PlayStation console timed exclusive, meaning that it cannot appear on another console until the timed exclusivity is up. Sifu has been out for a while on PlayStation 4, PlayStation 5, and PC, but it’s not yet available on either the Xbox Series X & S or Xbox One. |